| 上皮调节蛋白对表皮癌细胞A431生长的抑制机制 |
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| 引用本文： | 席全胜 潘巍. 上皮调节蛋白对表皮癌细胞A431生长的抑制机制[J]. Acta biochimica et biophysica Sinica, 2000, 32(6): 601-604 |
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| 作者姓名： | 席全胜 潘巍 |
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| 作者单位： | 中国科学院上海生物化学研究所,上海 |
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| 摘 要： | 对重组蛋白上皮调节蛋白（ｅｐｉｒｅｇｕｌｉｎ，ＥＰＩ）抑制表皮癌细胞Ａ４３１的生长机制进行了初步的探讨。通过Ｎｏｒｔｈｅｒｎ杂交发现，细胞受到重组蛋白质刺激后，其周期蛋白激酶的抑制因子ｐ２１＾ＷＡＦ１／ＣＩＰ１的ｍＲＮＡ含量有较明显的提高。报告基因分析表明，ｐ２１＾ＷＡＦ１／ＣＩＰ１启动子区的转录因子ＳＴＡＴ１结合序列对ＥＰＩ的刺激有较强的反应。流式细胞仪的测定则表明，在ＥＰＩ导致的细胞生长抑制中
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| 关 键 词： | 生长抑制 STAT1 凋亡 表皮癌细胞 |
On the Mechanism of Growth Inhibition of Epiregulin in A431 Epidermal Carcinoma Cells |
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| XI Quan Sheng,PAN Wei,ZHANG Qian,QIAN Xin Guo,LI Zai Ping,GAN Ren Bao. On the Mechanism of Growth Inhibition of Epiregulin in A431 Epidermal Carcinoma Cells[J]. Acta biochimica et biophysica Sinica, 2000, 32(6): 601-604 |
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| Authors: | XI Quan Sheng PAN Wei ZHANG Qian QIAN Xin Guo LI Zai Ping GAN Ren Bao |
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| Affiliation: | XI Quan Sheng,PAN Wei,ZHANG Qian,QIAN Xin Guo,LI Zai Ping,GAN Ren Bao * |
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| Abstract: | Preliminary investigation on the mechanism of the gr owth inhibition by recombinant epiregulin(EPI)of epidermal carcinoma cell A431 i s reported. Northern blotting indicated that the mRNA level of cyclin dependent kinase(CDK)inhibitor, p21 WAF1/CIP1 , was increased significantly after stim ulation of the recombinant epiregulin protein. Luc reporter revealed that STAT1 could bind the promoter region of p21 in response to the EPI signal. Flow cytome try assay showed that the EPI induced growth inhibition was not related to the apoptosis. The above results indicate that the EPI induced cell growth inhibiti on might result from the STAT1 stimulated expression of p21, leading to the G1 arrest. |
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| Keywords: | growth inhibition p21 WAF1/CIP1 STAT1 apop tosis |
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