Micropropagation with 3-methyleneoxindole: Its obligatory role in indole-3-acetic acid-mediated auxin action |
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Authors: | V K Tuli |
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Institution: | (1) Department of Plant Science, Natural Science Research, 1210 E. 223rd Street Bldg 322, 90745 Carson, CA |
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Abstract: | Summary 3-Methyleneoxindole (MO), a metabolite of the plant auxin indole-3-acetic acid (IAA), was more active than IAA in supporting
Stage II and III micropropagation of several plant species. In Stage II micropropagation, characterized by the rapid numerical
increase of shoots, the optimal IAA concentration was 0.01 mM compared to 0.1 mM MO for most plants. In Stage III micropropagation
where auxin is required for the rhizogenic response, 0.1 μM MO was more effective than 0.01 mM IAA. Inhibition analysis of
plant growth with chlorogenic acid (CGA) suggested an obligatory role for MO in IAA-mediated auxin reactions: CGA, which blocks
the enzymatic oxidation of IAA to MO, in vivo, completely abolished IAA's ability to support the growth of explants during
micropropagation. In contrast, CGA did not inhibit the auxin activity of MO, the product of the blocked reaction. The growth
rate and rooting efficiency of tobacco propagules in Stage III medium was improved substantially if these were first exposed
to a high concentrations of MO and subsequently transferred to media containing low or no MO. |
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Keywords: | auxin activity IAA oxidation product chlorogenic acid inhibition analysis |
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