A method for direct soil extraction and PCR amplification of endomycorrhizal fungal DNA |
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| Authors: | V. P. Claassen R. J. Zasoski B. M. Tyler |
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| Affiliation: | (1) Soils and Biogeochemistry Section, Department of Land, Air, and Water Resources, University of California Davis, Davis, CA 95616, USA e-mail: vpclaassen@ucdavis.edu, US;(2) Department of Plant Pathology, University of California Davis, Davis, CA 95616, USA, US |
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| Abstract: | DNA from endomycorrhizal fungi was extracted directly from a weathered soil (alfisol) mixed with sand. Mycorrhizae were established in a greenhouse culture of Glomus clarum with Sudan grass (Sorghum vulgare var. sudanense) host plants. The extraction procedure included enzymatic digestion of cell walls, sodium dodecyl sulfate lysis of cells, polyvinylpolypyrrolidone absorption of organic compounds, and ethanol precipitation of the DNA. DNA in the extracts was amplified by the polymerase chain reaction using primers from the nuclear 17S rRNA sequence that were general to fungi or were specific to endomycorrhizae. Accepted: 17 July 1996 |
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| Keywords: | Endomycorrhizae DNA rRNA PCR Direct soil extraction |
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