| 阿魏酸钠诱导分化的PC12细胞裂解液的无细胞滤液的抗抑郁样效果 |
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| 引用本文： | 廖铭能,于立坚,张永平,马润娣,张霄瑜,于廷曦.阿魏酸钠诱导分化的PC12细胞裂解液的无细胞滤液的抗抑郁样效果[J].细胞生物学杂志,2011(6). |
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| 作者姓名： | 廖铭能 于立坚 张永平 马润娣 张霄瑜 于廷曦 |
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| 作者单位： | 广东海洋大学海洋药物重点实验室;Department of Otorhinolaryngology-Head and Neck Surgery;University of Maryland School of Medicine;MD 21228;Cell Biology Group;Department of Surgery;Department of Pathology;University of Maryland School of Medicine and Baltimore Veterans Affairs Medical Center;MD 21201; |
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| 基金项目： | 广东海洋大学研究生基金资助项目~~ |
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| 摘 要： | 阿魏酸(ferulic acid,FA)是一种广泛存在的低毒酚酸,阿魏酸钠(sodium ferulate,SF)则是其钠盐。先前的研究已经证实,阿魏酸钠具有显著的神经保护和神经发生增强作用及抗抑郁效果。该研究的目的在于探讨阿魏酸钠诱导分化的PC12细胞裂解液的无细胞滤液可能的抗抑郁效果。PC12细胞在含80μmol/L阿魏酸钠的DMEM培养基中孵育6d,无菌条件下制备阿魏酸钠诱导分化的PC12细胞液的无细胞滤液,测定PC12细胞裂解液无细胞滤液中残留的阿魏酸钠量。以慢性不可预期的多种刺激制造大鼠抑郁模型,用行为学、形态学、免疫组织化学和BrdU掺入等方法观察并检测阿魏酸钠诱导分化的PC12细胞裂解液的无细胞滤液对慢性应激大鼠抑郁模型行为学、海马的组织病理学、海马和大脑皮质的神经生长因子(nerve growth factor,NGF)及脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)的表达及神经发生的影响。实验证实,阿魏酸钠诱导分化的PC12细胞裂解液的无细胞滤液能改善抑郁症样模型大鼠的行为学障碍,上调其海马和大脑皮质NGF和BNDF的表达,增加海马神经干细...
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| 关 键 词： | 阿魏酸钠 PC12细胞 诱导分化 细胞裂解液 抗抑郁样效果 慢性抑郁模型 大鼠 |
Antidepressant-like Effect of Cell-free Filtrate of Sodium Ferulate-induced and Differentioned PC12 Cell Lysates |
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| Ming-Neng Liao,Li-Jian Yu,Yong-Ping Zhang,Run-Di Ma,Xiao-Yu Zhang,Ting-Xi Yu.Antidepressant-like Effect of Cell-free Filtrate of Sodium Ferulate-induced and Differentioned PC12 Cell Lysates[J].Chinese Journal of Cell Biology,2011(6). |
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| Authors: | Ming-Neng Liao Li-Jian Yu Yong-Ping Zhang Run-Di Ma Xiao-Yu Zhang Ting-Xi Yu |
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| Institution: | Ming-Neng Liao~1,Li-Jian Yu~(1*),Yong-Ping Zhang~1,Run-Di Ma~(1*),Xiao-Yu Zhang~(1,3),Ting-Xi Yu~(1,2*) (1 Key Laboratory of Marine Materia Medica,Guangdong Ocean University,Zhanjiang 524025,China,2 Cell Biology Group,Department of Surgery,Department of Pathology,University of Maryland School of Medicine and Baltimore Veterans Affairs Medical Center,Baltimore,MD 21201,USA,3 Department of Otorhinolaryngology-Head and Neck Surgery,University of Maryland School of Medicine,MD 21228,USA) |
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| Abstract: | Ferulic acid(FA),3-(4-hydroxy-3-methoxyphenyl)-2-propenoic acid,is one of the most common phenolic acids with low toxicity,and sodium ferulate(SF) is its sodium salt.Our previous work demonstrates that SF has significant neuroprotective and neurogenesis-enhancing actions and antidepressant-like effects.The aim of this study was to investigate a potential antidepressant-like effect of cell-free filtrate of sodium ferulate-induced and differentioned PC 12 cell lysates(SFIDPC12CL) in the chronic mild stress(CM... |
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| Keywords: | sodium ferulate PC12 cells induction of differentiation cell lysates antidepressant-like effect chronic mild stress rats |
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