| 小鼠动物实验方法系列专题(五) 应用高架十字迷宫分析小鼠焦虑行为 |
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| 引用本文： | 王维刚,吴文婷,周嘉斌,严惠敏.小鼠动物实验方法系列专题(五) 应用高架十字迷宫分析小鼠焦虑行为[J].细胞生物学杂志,2011(5). |
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| 作者姓名： | 王维刚 吴文婷 周嘉斌 严惠敏 |
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| 作者单位： | 同济大学化学系;同济大学生命科学与技术学院;上海南方模式生物研究中心; |
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| 基金项目： | 国家863计划(No.2008AA02Z126); 上海市科委科技创新行动计划(No.10140901400)资助项目~~ |
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| 摘 要： | 高架十字迷宫(the elevated plus maze,EPM)是研究小鼠焦虑行为的重要实验,本文介绍了EPM的原理和实验步骤。将小鼠置于开臂闭臂接合处,面向开臂,通过录像记录分析小鼠在EPM的表现。结果发现,两种小鼠均可成功完成实验,C57小鼠在EPM内较不活跃,闭臂滞留时间占总时间百分比显著高于129Sv小鼠。应用EPM可以简单直观地分析小鼠焦虑行为。
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| 关 键 词： | 高架十字迷宫 焦虑 行为学 小鼠 |
The Use of the Elevated Plus Maze to Analyze Anxiety Behavior in Mice |
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| Wei-Gang Wang,Wen-Ting Wu,Jia-Bin Zhou,Hui-Min Yan.The Use of the Elevated Plus Maze to Analyze Anxiety Behavior in Mice[J].Chinese Journal of Cell Biology,2011(5). |
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| Authors: | Wei-Gang Wang Wen-Ting Wu Jia-Bin Zhou Hui-Min Yan |
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| Institution: | Wei-Gang Wang~(1,2),Wen-Ting Wu~3,Jia-Bin Zhou~3,Hui-Min Yan~(3*) (1 Department of Chemistry,Tongji University,Shanghai 200092,China,2 School of Life Science and Technology,3 Shanghai Research Center for Model Organisms,Shanghai 201203,China) |
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| Abstract: | The elevated plus maze(EPM) is an important behavioral assay for rodents to assess the anxiety behavior.We introduced the basic principle and protocol of EPM.Mice are placed at the junction of the four arms facing the same open arm,activities were recorded by a video-tracking system.Both finished the test,but C57 showed less general activity than 129Sv.C57 mice showed significantly higher percent of closed/total time than the 129Sv.EPM is a simple and direct test to analyze anxiety behavior in mice. |
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| Keywords: | the elevated plus maze anxiety ethology mouse |
| 本文献已被 CNKI 等数据库收录！ |
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