| 小鼠脑室内注射神经干细胞裂解液促进谷氨酸盐诱导的兴奋性神经元损伤的修复 |
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| 引用本文： | 于立坚,马娟,马润娣,张永平,房娟芝,张霄瑜,于廷曦.小鼠脑室内注射神经干细胞裂解液促进谷氨酸盐诱导的兴奋性神经元损伤的修复[J].细胞生物学杂志,2011(10). |
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| 作者姓名： | 于立坚 马娟 马润娣 张永平 房娟芝 张霄瑜 于廷曦 |
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| 作者单位： | 广东海洋大学海洋药物重点实验室;佛山德溥生物医药科技有限公司;Department of Otorhinolaryngology-Head and Neck Surgery;University of Maryland School of Medicine;Cell Biology Group;Department of Surgery;Department of Pathology;University of Maryland School of Medicine and Baltimore Veterans Affairs Medical Center; |
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| 基金项目： | 广东海洋大学研究生基金资助项目~~ |
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| 摘 要： | 先前的研究已经证实,阿魏酸钠诱导分化的PC12细胞裂解液的无细胞滤液具有改善抑郁症样模型大鼠的行为学障碍、上调其海马和大脑皮质神经生长因子(nerve growth factor,NGF)和脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)的表达、增加海马神经干细胞(neural stem cells,NSCs)/神经前体细胞(neural progenitor cells)增殖的效果。该研究的目的在于探讨神经干细胞裂解液的无细胞滤液(cell-free filtrate ofneural stem cell lysates,FNSCL)脑室内注射促进谷氨酸盐诱导的成年小鼠兴奋性神经元损伤修复的可能性。成年小鼠谷氨酸单钠(monosodiumglutamate,MSG,2.0g/(kg·d))灌胃,连续10日,造成兴奋性神经元损伤模型。自孕15 d的昆明种小鼠取胎脑,分离、培养神经干细胞,免疫细胞化学法检测巢蛋白(nestin)抗原,制备神经干细胞裂解液的无细胞滤液。MSG+NSCs组动物在MSG灌胃后接收脑室内NSCs移植,MSG+FNSCL组动物在MSG灌胃...
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| 关 键 词： | 脑修复 神经干细胞裂解液 神经干细胞因子 神经干细胞 兴奋性毒性脑损伤 小鼠 |
Repair Following Glutamate-induced Excitotoxic Neuronal Damage Mediated by Intracerebroventricular Injection of Cell-free Filtrate of Neural Stem Cell Lysates in Adult Mice |
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| Yu Lijian,Ma Juan,Ma Rundi,Yu Tingxi.Repair Following Glutamate-induced Excitotoxic Neuronal Damage Mediated by Intracerebroventricular Injection of Cell-free Filtrate of Neural Stem Cell Lysates in Adult Mice[J].Chinese Journal of Cell Biology,2011(10). |
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| Authors: | Yu Lijian Ma Juan Ma Rundi Yu Tingxi |
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| Institution: | Yu Lijian~(1,4*),Ma Juan~1,Ma Rundi~(1,Zhang Yongping~1,Fang Juanzhi~1,Zhang Xiaoyu~(1,3,4),Yu Tingxi~(1,2,4*) (1 Key Laboratory of Marine Materia Medica,Guangdong Ocean University,Zhanjiang 524025,China,2 Cell Biology Group,Department of Surgery,Department of Pathology,University of Maryland School of Medicine and Baltimore Veterans Affairs Medical Center,Baltimore,MD 21201,USA,3 Department of Otorhinolaryngology-Head and Neck Surgery,University of Maryland School of Medicine,MD 21228,... |
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| Abstract: | Our previous study demonstrated that cell-free filtrate of sodium ferulate-induced and differen-tioned PC12 cell lysates significantly attenuated chronic mild stress-induced depression-like behavioural disorders, up-regulated hippocampal and cerebral cortex expressions of nerve growth factor(NGF) and brain-derived neurotrophic factor(BDNF),and increased hippocampal number of neural stem cells(NSC)/neural progenitor cells in mice.The present study was undertaken to investigate the possibility of the repair f... |
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| Keywords: | brain repair filtrate of neural stem cell lysates neural stem cell factors neural stem cells excitotoxic brain injury mice |
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