Marker assisted pea breeding: <Emphasis Type="Italic">eIF4E</Emphasis> allele specific markers to <Emphasis Type="Italic">pea seed-borne mosaic virus</Emphasis> (PSbMV) resistance |
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Authors: | Petr Smýkal Dana Šafářová Milan Navrátil Radmila Dostalová |
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Institution: | 1.Department of Plant Biotechnology,Agritec Plant Research Ltd,?umperk,Czech Republic;2.Department of Cell Biology and Genetics, Faculty of Science,Palacky University in Olomouc,Olomouc,Czech Republic;3.Grain Legumes Department,Agritec Plant Research Ltd,?umperk,Czech Republic |
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Abstract: | Traditional plant breeding relies upon crosses and subsequent selection of genotypes to meet desirable traits. The incorporation
of marker-assisted selection into breeding strategies would result in a reduction in the number of offspring to be propagated,
selected and tested. In the case of pea (Pisum sativum L.), the testing of resistance to viral pathogens such as pea seed-borne mosaic virus (PSbMV) is included in the breeding process. Resistance to the common strains of PSbMV is conferred by a single recessive
gene (eIF4E), localized on LG VI (sbm-1 locus). We have analyzed for variation in the eIF4E genomic sequences from 43 pea varieties and breeding lines, reported as donors of resistance. This enabled a comprehensive
investigation of the eIF4E gene structure and mutations responsible for PSbMV resistance were identified. Subsequently, PCR-based and gene-specific
single nucleotide polymorphism and co-dominant amplicon length polymorphism markers were developed. All together 60 accessions
were analyzed using sequence data and/or allele specific DNA markers. Developed allele specific markers were reproducibly
amplified across a broad spectra of pea varieties and breeding lines. These were found to be 100% accurate in detecting the
presence of the respective alleles when compared to symptomology and ELISA, testing (74% reliable). Hence, these molecular
markers will substantially speed-up PSbMV diagnosis and resistance breeding processes in pea. |
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