| 人外周血淋巴细胞体外诱导培养前后细胞表型与细胞毒活性相关性研究 |
| |
| 引用本文： | 刘运洪,孟明耀,解燕华,刘莹,魏传钰,侯宗柳. 人外周血淋巴细胞体外诱导培养前后细胞表型与细胞毒活性相关性研究[J]. 细胞生物学杂志, 2011, 0(5) |
| |
| 作者姓名： | 刘运洪 孟明耀 解燕华 刘莹 魏传钰 侯宗柳 |
| |
| 作者单位： | 昆明医学院附属延安医院中心实验室; |
| |
| 基金项目： | 昆明市科技局重点项目(No.08S090203); 昆明医学院研究生创新基金(No.2009J10)资助项目~~ |
| |
| 摘 要： | 为了分析体外细胞因子诱导培养CIK细胞过程中细胞表型的变化与其杀瘤活性的相关性及为临床过继免疫治疗提供实验依据,本研究采用体外诱导方法扩增培养正常人外周血淋巴细胞及单个核细胞,应用流式细胞术测定培养前、培养第7天和第14天的CD3~+等15种不同表型细胞百分率的变化,用CCK-8试剂检测第7天和第14天的细胞毒活性。结果显示,扩增培养后T细胞活化表型的表达和细胞毒活性在第7天最强,与其细胞表型CD3~+CD25~+、CD3~+CD28~+、CD3~+CD25~+CD28~+、CD3~+CD4~+呈正相关(P<0.05),与CD3~+CD45RA~+CD45RO~+呈负相关(P<0.05)。本研究表明测定培养细胞活化相关表型可以间接监测其杀瘤能力,为临床CIK细胞过继免疫治疗的应用提供实验依据。
|
| 关 键 词： | 体外诱导 细胞表型 细胞毒活性 CIK |
The Correlation Between Phenotypes of Human Peripheral Blood Lymphocytes Induce-cultured in vitro and Cytotoxic Activity |
| |
| Yun-Hong Liu,Ming-Yao Meng,Yan-Hua Xie,Ying Liu,Chuan-Yu Wei,Zong-Liu Hou. The Correlation Between Phenotypes of Human Peripheral Blood Lymphocytes Induce-cultured in vitro and Cytotoxic Activity[J]. Chinese Journal of Cell Biology, 2011, 0(5) |
| |
| Authors: | Yun-Hong Liu Ming-Yao Meng Yan-Hua Xie Ying Liu Chuan-Yu Wei Zong-Liu Hou |
| |
| Affiliation: | Yun-Hong Liu,Ming-Yao Meng,Yan-Hua Xie,Ying Liu,Chuan-Yu Wei,Zong-Liu Hou~* (Key Lab of Kunming Medical College Affiliated Yan'an Hospital,Kunming 650051,China) |
| |
| Abstract: | In order to analyze the correlation between the phenotypes of in vitro cultured CIK cells and the cytotoxic activity,normal human peripheral blood lymphocytes and mononuclear cells were cultured and the cell percentage variation of 15 different phenotypes was measured by flow cytometry at the culture time points 0,7 and 14 days.In addition,the cytotoxic activity was tested with CCK-8 reagent.The results indicated that the expression of T cell active phenotypes after amplification and its cytotoxic activity ... |
| |
| Keywords: | cytokine-induced-culture phenotype cytotoxic activity CIK |
| 本文献已被 CNKI 等数据库收录！ |
|
