Restriction fragment map of sugar beet (Beta vulgaris L.) chloroplast DNA |
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| Authors: | Yuji Kishima Tetsuo Mikami Takeo Harada Kazuo Shinozaki Masahiro Sugiura Toshiro Kinoshita |
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| Affiliation: | (1) Plant Breeding Institute, Faculty of Agriculture, Hokkaido University, 060 Sapporo, Japan;(2) Center for Gene Research, Nagoya University, Chikusa, 464 Nagoya, Japan;(3) Present address: Hokkaido Central Agricultural Experiment Station, 069-13 Naganuma, Japan |
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| Abstract: | Summary A restriction endonuclease fragment map of sugar beet chloroplast DNA (ctDNA) has been constructed with the enzymes SmaI, PstI and PvuII. The ctDNA was found to be contained in a circular molecule of 148.5 kbp. In common with many other higher plant ctDNAs, sugar beet ctDNA consists of two inverted repeat sequences of about 20.5 kbp separated by two single-copy regions of different sizes (about 23.2 and 84.3 kbp). Southern hybridization analyses indicated that the genes for rRNAs (23S+16S) and the large subunit of ribulose 1,5-bisphosphate carboxylase were located in the inverted repeats and the large single-copy regions, respectively. |
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| Keywords: | Beta vulgaris chloroplast DNA electron microscopy restriction fragment map sugar beet |
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