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Design of flow chamber with electronic cell volume capability and light detection optics for multilaser flow cytometry
Authors:W H Schuette  S E Shackney  F A Plowman  H W Tipton  C A Smith  M A MacCollum
Abstract:A multibeam optical detection system has been developed with a high optical efficiency, achieved through a reduction in the number of optical interfaces employed in the system. This reduction is made possible by a combination of employing simple lenses, gluing the objective lens directly upon the face of the flow cuvette and the extraction of only one fluorescence signal from each laser beam. A modified flow chamber is also described that includes fluidic resistance elements for the elimination of most of the electric shielding normally associated with electronic cell volume measurements.
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