朱砂根愈伤组织培养及悬浮细胞系建立

以朱砂根(Ardisia crenata Sims.)无菌苗的茎段、叶片、胚轴和胚根为外植体进行愈伤组织诱导研究。结果表明:胚根在含有2,4-D的培养基中的诱导率最高,在添加5 mg L-1 AgNO3的MS+2,4-D 0.5 mg L-1+KT 0.01 mg L-1培养基中继代培养的增殖系数高达8倍以上。培养中获得了5种类型的愈伤组织(I-白色湿软状、Ⅱ-白色冰砂状、Ⅲ-淡黄色颗粒状、Ⅳ-黄绿色块状和V-绿色块状),其中Ⅱ和Ⅲ型愈伤组织可以成功建立悬浮细胞系,用M S+2,4-D 0.5 mg L-1+KT 0.01 mg L-1培养基进行固-液轮回培养,可以较好地保持悬浮细胞系。

Callus Induction and Establishment of Suspension Cell Line of Ardisia crenata Sims.

Stems, leaves, hypocotyls and radicles from aseptic seedlings of Ardisia crenata Sims. used as explants were cultured on different mediums to induce callus. The results showed that radicles of A. crenata cultured on mediums with 2,4-D had the highest callus inducation rate. The callus subcultured on MS +2,4-D 0.5 mg L^-1+KT 0.01 mg L^-1 supplemented with 5 mg L^-1AgNO₃ had more than 8 times proliferation coefficient. Five types of callus, such as white, wet and soft callus (I), white gibraltar-like callus (II), yellow granular callus (III), yellow green nubbly callus (IV), and green nubbly callus (V) were obtained. Among which callus II and III could successfully establish suspension cell system, and the callus could be well maintained cultured on MS+2,4-D 0.5 mg L^-1+KT 0.01 mg L^-1 by solid-liquid transition.