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Two-step one-dimensional SDS-PAGE analysis of LMW subunits of glutelin
Authors:R. B. Gupta  K. W. Shepherd
Affiliation:(1) Department of Agronomy, Waite Agricultural Research Institute, University of Adelaide, 5064 Glen Osmond, SA, Australia;(2) Present address: Wheat Research Unit, CSIRO-Division of Plant Industry, P.O. Box 7, 2113 North Ryde, NSW, Australia
Abstract:Summary Analysis of intergeneric substitution lines in hexaploid wheats by a two-step electrophoretic method of protein separation revealed that low-molecular-weight (LMW) subunits of glutelin in Triticum longissimum, T. Umbelullatum, Elytrigia elongata (2 x) were controlled by chromosomes/chromosome arms 1Sl, 1U, and 1ES, respectively. A LMW glutelin band in Secale montanum was detected but its chromosomal location could not be determined. Genes controlling gliadins and HMW subunits of glutelin were also located on chromosome 1Sl in T. longissimum.The term lsquoglutelinrsquo refers to the polymeric prolamins of cereals, e.g., glutenins in wheat, HMW, and 75-k secalins in rye
Keywords:Triticum longissimum  T. umbelullatum  Elytrigia elongata  LMW subunits of glutelin  Chromosomal location
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