Two-step one-dimensional SDS-PAGE analysis of LMW subunits of glutelin |
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Authors: | R. B. Gupta K. W. Shepherd |
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Affiliation: | (1) Department of Agronomy, Waite Agricultural Research Institute, University of Adelaide, 5064 Glen Osmond, SA, Australia;(2) Present address: Wheat Research Unit, CSIRO-Division of Plant Industry, P.O. Box 7, 2113 North Ryde, NSW, Australia |
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Abstract: | Summary Analysis of intergeneric substitution lines in hexaploid wheats by a two-step electrophoretic method of protein separation revealed that low-molecular-weight (LMW) subunits of glutelin in Triticum longissimum, T. Umbelullatum, Elytrigia elongata (2 x) were controlled by chromosomes/chromosome arms 1Sl, 1U, and 1ES, respectively. A LMW glutelin band in Secale montanum was detected but its chromosomal location could not be determined. Genes controlling gliadins and HMW subunits of glutelin were also located on chromosome 1Sl in T. longissimum.The term glutelin refers to the polymeric prolamins of cereals, e.g., glutenins in wheat, HMW, and 75-k secalins in rye |
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Keywords: | Triticum longissimum T. umbelullatum Elytrigia elongata LMW subunits of glutelin Chromosomal location |
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