脊髓薄片器官型培养的方法研究（简报）

脊髓的器官培养技术是借助体外培养技术，将脊髓或其一部分分离出来进行培养、研究的技术。因其保留有脊髓神经元及其周围的组织结构，与体内的生理环境相似，是探讨脊髓形态发生、构筑特点、生理功能及病理改变等问题的一条重要途径，在近年来得到飞速发展，成为国际神经科领域的一大研究热点。本文旨在利用脊髓薄片器官型培养技

A study on organotypic spinal cord slice culture]

This study was aimed at developing a method of organotypic culture of spinal cord slice. The slice culture was prepared using lumbar spinal cord from 8-day-old rat. The survival of alpha-motor neuron was evaluated by the morphological observation and by monoclonal antibody SMI-32, a nonphosphorylated neurofilament marker and immunohistochemical staining. Lactate dehydrogenase (LDH) level in culture medium was also measured. The result showed that the spinal cord explant could be maintained in the culture for more than 2 months with excellent cellular organization and stable population of ventral motor neurons. The level of LDH at different culture times had no significant difference. Organotypic spinal cord slice culture may provide an effective method for studying physiological and pathological changes, and neuroprotection of spinal cord.