Functional diversity and community structure of microorganisms in rhizosphere and non-rhizosphere Canadian arctic soils |
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Authors: | L Tam AM Derry PG Kevan JT Trevors |
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Institution: | (1) Department of Environmental Biology, University of Guelph, Ontario, Canada, N1G 2WL;(2) Department of Biological Sciences, University of Alberta, Edmonton, Canada, AB T6F 2E9 |
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Abstract: | Functional diversities of microorganisms in arctic soil samples at three incubation temperatures were assessed using sole-carbon-source-utilization (SCSU). Soil samples from four sites were collected from the rhizosphere and non-rhizosphere soils. Microorganisms were extracted from samples and inoculated into ECO-Biolog plates and incubated at 4, 10 and 28 °C. Calculations of Shannon–Weaver diversity and Shannon–Weaver evenness were based on the substrate utilization in the Biolog plates. Shannon–Weaver diversities (H ) in rhizosphere samples were significantly greater (
H = 3.023 ± 0.197; P < 0.005) than in non-rhizosphere samples (
H = 2.770 ± 0.154). Similarly, the evenness (E) of the inoculated microbial cells exhibited significant differences (P < 0.005) between the rhizosphere and non-rhizosphere soil samples (
E = 0.880 ± 0.057 for soils with rhizosphere;
E = 0.807 ± 0.044 for non-rhizosphere samples). Higher microbial diversity and evenness were observed in samples incubated at 4 °C than at 28 °C least significant difference (lsd) = 0.29], and evenness indices were higher in rhizosphere samples than in non-rhizosphere soils incubated at all three temperatures (lsd = 0.02). Principal component analysis (PCA) of the multivariate data set differentiated the soil samples on the relatively gross scale of microbial communities isolated from rhizosphere and non-rhizosphere soils at all three temperatures. |
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Keywords: | Arctic biodiversity Biolog Canada community structure microorganisms non-rhizosphere soil rhizosphere soil |
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