Effects of dietary manganese on arterial glycosaminoglycan metabolism in sprague—dawley rats |
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Authors: | Peiying Yang Dorothy J Klimis-Tavantzis |
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Institution: | (1) Department of Food Science and Human Nutrition, University of Maine, Orono, Maine |
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Abstract: | The objectives of this study were to determine whether dietary manganese deficiency alters total glycosaminoglycan (GAG) concentration
and composition and glycosyltransferase activity in rat aortas. Sprague-Dawley rats were fed either a manganese-deficient
or a manganese-sufficient diet. Arterial GAGs were isolated and quantified by measuring uronic acid content. The individual
GAGs were separated and quantified with cellulose acetate electrophoresis. The activity of the enzyme galactosyltransferase
I was measured using a 100,000g particulate fraction and 4-methylumbelliferylxyloside (Xyl-MU) as an acceptor. There was a significant decrease (p <- 0.05) in uronic acid content in the manganese-deficient (1.18 ± 0.08 mg/g) rat aortas as compared with the manganese-sufficient
(1.59 ± 0.10 mg/g) ones. Chondroitin sulfate and heparan sulfate concentrations were decreased by 38% (p < 0.01) and 36% (p < 0.05), respectively, in the manganese-deficient rat aortas. The incorporation of UDP-galactose to acceptors by the manganese-deficient
rat aorta preparations was increased by 28% as compared to the manganese-sufficient preparations. These results indicate that
manganese is involved in arterial GAG metabolism by affecting the enzyme galactosyltransferase and that changes in GAG concentration
and composition with manganese deficiency may ultimately affect arterial wall integrity and subsequently cardiovascular health.
This is the first work to demonstrate that manganese nutrition is important in arterial GAG metabolism. |
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Keywords: | Manganese glycosaminoglycans aorta Sprague-Dawley rats glycosyltransferase |
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