| Abstract: | The heptacosapeptide amide corresponding to the entire amino acid sequence of chicken secretin was synthesized by assembling four peptide fragments followed by deprotection with thioanisole-mediated trifluoromethanesulfonic acid in TFA. The deprotected peptide was purified by gel-filtration on Sephadex G-25, followed by ion-exchange chromatographies on CM-cellulose and DEAE-Sepharose. Synthetic peptide stimulated the flow of pancreatic alkaline juice and decreased systemic blood pressure in rats. |
