Stage-specific expression of fuco-neolacto- (Lewis X) and ganglio-series neutral glycosphingolipids during brain development: characterization of Lewis X and related glycosphingolipids in bovine,human and rat brain |
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Authors: | Somsankar Dasgupta Edward L Hogan Samuel S Spicer |
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Institution: | (1) Department of Neurology, Medical University of South Carolina, 171 Ashley Avenue, 29425-2232 Charleston, SC, USA;(2) Department of Pathology and Laboratory Medicine, Medical University of South Carolina, 171 Ashley Avenue, 29425-2232 Charleston, SC, USA |
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Abstract: | We have purified and characterized a bovine brain pentaglycosylceramide as Lewis X and identified it in human and rat brain using anti-Lewis X (anti-SSEA 1) monoclonal antibody. Neutral glycosphingolipid expression in developing rat brain has been examined by digoxigenin immunostaining and TLC-immunostaining using anti-SSEA 1 and anti-GgOse4Cer (GA1) monoclonal antibodies. Five transient Lewis X-series bands were identified in brain at embryonic day 15 that disappear by postnatal day 5 (one disappears at embryonic day 18). Gangliotetraosylceramide (GA1) first appears at embryonic day 21 and increases in concentration with age until postnatal day 21. In addition, we have purified another minor brain neutral glycosphingolipid and tentatively identified it as a Lewis X-series glycolipid by gas chromatography-mass spectrometry analysis followed by TLC-immunostaining with anti-SSEA 1 antibody.Abbreviations Cer
Ceramide, GlcCer, Glc 1-1Cer
- LacCer
Gal 1-4GlcCer
- CTH
Gal 1-4LacCer
- nLcOse4Cer
Gal 1-4GlcNAc 1-3LacCer
- nLcOse5Cer
Gal 1-3nLcOse4Cer
- GgOse4Cer
Gal 1-3GalNAc 1-4LacCer
- DPA
diphenylamine-aniline-phosphoric acid
- SSEA
stage-specific embryonic antigen
- NGSL
neutral glycosphingolipid
- TLC
thin-layer tomography
- HPTLC
high performance thin-layer chromatography
- GA1
gangliotetraosyleramide
- SAT-2
sialytransferase-2
- GalNAcT-1
galactosaminyltransferase-1
- DIG-IS
digoxigenin-immunostaining
- PMAAS
partially methylated alditol acetates
- DCE
dichloroethane
- TLC-IS
TLC-immunostaining
- (Lex)
Lewis X
- NK
murine natural killer |
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Keywords: | neutral glycosphingolipid brain development stage-specific expression gas chromatography-mass spectrometry |
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