Sunflower seed oil and oleic acid utilization for the production of rhamnolipids by Thermus thermophilus HB8 |
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Authors: | Anastasia A Pantazaki Maria I Dimopoulou Olga M Simou Agathi A Pritsa |
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Institution: | (1) Laboratory of Biochemistry, Department of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece;(2) Department of Nutrition and Dietetics, Faculty of Food Technology and Nutrition, Alexander Technological Educational Institute of Thessaloniki, PO Box 141 Gr, 57400 Sindos, Greece |
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Abstract: | The potential production of rhamnolipids was demonstrated using the thermophilic eubacterium Thermus thermophilus HB8 and sunflower seed oil or oleic acid as carbon sources. Sunflower seed oil was directly hydrolyzed by secretion of lipase
and became a favorable carbon source for rhamnolipids production. Rhamnolipids levels were attainted high values, comparable
to those produced by Pseudomonas strains from similar sources. Rhamnolipids synthesis in oleic acid exhibited a long period of induction, while in sunflower
seed oil, the synthesis is more rapid. Glucose resulted in a more protracted period of rhamnolipids production after exhaustion
of each or both carbon sources. Both mono- and di-rhamnolipids were identified by thin-layer chromatography (TLC) in the total
rhamnolipids extract. The molecular composition of the produced biosurfactant was evaluated by Fourier transform infrared
(FTIR) and attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy and LC-MS analysis. Furthermore,
secretion of rhamnolipids was confirmed on agar plates. The antimicrobial activity of rhamnolipids was detected against the
bacterium Micrococcus lysodeikticus using a lysoplate assay. These results demonstrate that rhamnolipids produced in these substrates can be useful in both environmental
and food industry applications by using cheap oil wastes. The alternative use of this thermophilic microorganism opens a new
perspective concerning the valorization of wastes containing plant oils or frying oils to reduce the cost of rhamnolipids
production. |
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