马蔺组织培养快繁技术体系研究

以成熟且具活力的马蔺种子为外植体，以MS为基本培养基，与不同浓度比例的2,4-D、BA、NAA、KT等植物生长调节剂构成愈伤组织诱导、分化、生根等培养基的组合方案，对马蔺组织培养快繁技术开展系统研究。结果表明，不同植物生长调节剂组合的培养基对愈伤组织的诱导率有显著影响，MS+2,4-D 4 mg·L^-1+BA2 mg·L^-1、MS+2,4-D 4 mg·L^-1+BA5 mg·L^-1和MS+2,4-D 2 mg·L^-1+KT1.5 mg·L^-1为最佳诱导培养基，诱导出愈率均在58%以上，显著高于其它培养基组合方案（p<0.05）；最佳分化培养基为MS+BA4 mg·L^-1和MS+BA1 mg·L^-1+NAA0.15 mg·L^-1，绿苗分化率高达100%，生长势好，状态叶和分化芽丛质量好；适宜继代增殖培养基为MS+BA2 mg·L^-1+NAA0.1 mg·L^-1、MS+BA1 mg·L^-1+NAA0.2 mg·L^-1；1/2MS为最适宜的生根培养基，1/2MS+IBA0.5 mg·L^-1、1/2MS+IBA0.5 mg·L^-1+NAA0.5 mg·L^-1两种组合也相对较好；试管苗不需炼苗可直接出瓶移栽于V_田土∶V_河沙=2∶1的土壤基质，成活率在95%以上。从而为马蔺提供了一套从“成熟种胚—诱导愈伤组织—绿苗分化—继代增殖—生根—试管苗移栽”等整个过程中各环节的最佳培养基和操作规程的组培快繁体系。

Technological System of Tissue Culture and Rapid Propagation of Iris lactea Pall.var.chinensis(Fisch.) Koidz.

Iris lactea Pall. var. chinensis(Fisch.) Kordz. is a kind of water-saving, ornamental, medicinal and fodder plants with excellent characteristics. In this paper, the mature seeds were used as the explants, and the combining medium schemes for inducing callus, differentiation and rooting, etc. were designed with different concentration of 2,4-D, NAA, BA, KT based on the MS culture medium, and the system of tissue culture and rapid propagation of I. lactea was studied. The results showed that the variable rat...