Noninvasive Demonstration of In Vivo 3-Fluoro-3-Deoxy-D-Glucose Metabolism in Rat Brain by 19F Nuclear Magnetic Resonance Spectroscopy: Suitable Probe for Monitoring Cerebral Aldose Reductase Activities |
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| Authors: | Ingrid L Kwee Tsutomu Nakada Peter J Card |
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| Institution: | Neurochemistry Research Laboratory, Veterans Administration Medical Center, Martinez and Department of Neurology, University of California, Davis, California;E. I. Du Pont de Nemours and Co., Diagnostic Imaging Research, Biomedical Products Department, North Billerica, Massachusetts, U.S.A. |
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| Abstract: | The metabolism of 3-fluoro-3-deoxy-D-glucose (3-FDG) in rat brain in vivo was investigated noninvasively using 19F nuclear magnetic resonance (NMR) Spectroscopy. Following an intravenous infusion of 3-FDG, 400 mg/kg, four resonances assigned to the α and β anomers of 3-FDG, 3-fluoro-3-deoxy-D-sorbitol, and 3-fluoro-3-deoxy-D-fructose were clearly resolved in brain, a result indicating that 3-FDG is metabolized primarily into the aldose reductase sorbitol (ARS) pathway. An orally administered aldose reductase inhibitor, sorbinil, caused reduction of the flux of 3-FDG into the ARS, an observation suggesting that the method can be applied in quantitative studies of ARS path way activities. Studies of 24-h urine specimens showed that in addition to the two metabolites observed in brain, F-was excreted into the urine. 3-FDG appears to be a suitable metabolic probe for assessing glucose metabolism in the ARS pathway by in vivo 19F NMR Spectroscopy. |
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| Keywords: | Nuclear magnetic resonance 3-Fluoro-3-deoxy-D-glucose Aldose reductase sorbitol pathway Glucose metabolism |
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