低能离子注入短命植物多伞阿魏(Ferula ferulaeoides)抗性生理特征

以能量30 keV、不同剂量N 离子注入多伞阿魏(Ferula ferulaeoides)种子用以研究多伞阿魏抗性生理指标的变化,以期为保护短命植物多伞阿魏提供理论依据.试验结果表明:随离子注入剂量增大,多伞阿魏种子发芽率和发芽指数逐渐下降;超氧化物歧化酶(SOD)活性增加、过氧化物酶(POD)、和过氧化氢酶(CAT)活性逐渐升高.剂量过大时,SOD、POD和CAT活性下降;游离脯氨酸含量逐渐降低,但在6×1016N /cm2剂量时含量升高;可溶性蛋白质含量先升高再降低,6×1016 N / cm2剂量时为最高值.通过对发芽率、发芽指数的结果进行方差分析,得出低剂量N 离子注入可破除多伞阿魏种子休眠,促进种子萌发;适当剂量N 离子注入可激活保护酶、脯氨酸和可溶性蛋白的表达.

Resistance physiology of ephemeral plant Ferula ferulaeoides irradiated by low-energy ion beam

The Ferula ferulaeoides seeds were irradiated using different dose of nitrogen ions with 30 KeV energy for studying the responses of resistance physiological indexes. The objective is to provide foundation to protect the ephemeral plant Ferula ferulaeoides in arid areas. Results showed that the irradiation with high dose of nitrogen ions significantly inhibited germination rate and germination index. The activities of superoxide dismutase (SOD), peroxidase (POD) and catalase(CAT) were enhanced with the increase of nitrogen ions. However, excessive nitrogen ions irradiating reduced the activities of SOD, POD and CAT. The peroxidase proline content fluctuated with increase of nitrogen ions irradiation. First it decreased, then increased and reached the peak at dose of 1×1016 N+/cm2, and then decreased. Besides, the soluble protein content significantly increased with the increase of nitrogen ions irradiation, reached the peak at doses of 6×1016N+/cm2, then decreased. By analyzing the variance of germination rate and germination index, the conclusion could be drawn the optimum dose of nitrogen ions irradiation could break seed dormancy, improve germination and enhance the activities of protective enzymes and content of proline and soluble protein.