The effect of continuous gamma irradiation on formation of sister chromatid exchanges (SCEs) and chromosomal aberrations in meristematic cells ofVicia faba |
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Authors: | P Kuglík Jana ?lotová Z Karpfel |
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Institution: | 1. Institute of Biophysics, Czechoslovak Academy of Sciences, Královopolská 135, 612 65, Brno, Czechoslovakia
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Abstract: | Germinated seeds ofVicia faba were continuously irradiated at low dose rate of gamma rays (0.05 Gy h-1) up to a total accumulated dose of 2 Gy. The FPG (fluorescence plus Giemsa) technique of differential chromatid staining
was used to monitor the frequency of sister chromatid exchanges (SCEs) in irradiated root tip meristem cells. The results
of the experiments have demonstrated that SCE frequency is raised by continuous gamma irradiation only in plant cells containing
BrdU in the chromosomal DNA. No effect concerning SCE formation was recorded at continuous irradiation of meristematic cells
of Vicia faba with native, i. e. BrdU-nonsubstituted, DNA. In contrast to SCEs, a significant increase was found in the yield
of chromosomal aberrations in all variants of irradiation. |
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