Purification and properties of polyphenoloxidase of mango peel (Mangifera indica) |
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Authors: | T N Prabha M V Patwardhan |
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Institution: | (1) Discipline of Fruit and Vegetable Technology, Central Food Technological Research Institute, 570 013 Mysore |
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Abstract: | Polyphenoloxidase from mango(Mangifera indica) peel was purified to homogeneity by ammonium sulphate fractionation, chromatography on DEAE-Sephadex and gel filtration of
Sephadex G-200. The enzyme had an apparent molecular weight of 136,000. Its pH and temperature optimum were 5.4 and 50‡C,
respectively. The enzyme possessed catecholase activity and was specific too-dihydroxy phenols. The enzyme also exhibited peroxidase activity. Some non-oxidizable phenolic compounds inhibited the enzyme
competitively. High inhibitory effects were also shown by some metal chelators and reducing agents, Mango peel polyphenol
oxidase when immobilized onto DEAE Sephadex showed slightly higher Km for catechol and lower pH and temperature optima. |
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Keywords: | Mango(Mangifera indica) peel polyphenoloxidase peroxidase immobilized enzyme |
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