Isolation and characterization of two middle repetitive DNA sequences of nuclear tobacco genome |
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| Authors: | V Kuhrová M Bezděk B Vyskot B Koukalová J Fajkus |
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| Institution: | (1) Institute of Biophysics, Czechoslovak Academy of Sciences, CS-612 65 Brno, Czechoslovakia |
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| Abstract: | Summary Two DNA sequences, R8.1 and R8.3, representing two distinct classes of tobacco genomic repeated DNA, were cloned and characterized by Southern blot analysis. Both R8.1 and R8.3 were found to be homologous to the Nicotiana tomentosiformis component of the allotetraploid Nicotiana tabacum genome, and each of them represents about 0.3% of nuclear DNA. The R8.1 and R8.3 differ in the mode of distribution in chromosomes, as revealed by in situ DNA/DNA hybridization. |
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| Keywords: | Repeated DNA Nicotiana tabacum Nicotiana tomentosiformis Hybridization in situ |
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