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Comparison of Groundnut bud necrosis virus isolates based on movement protein (NSm) gene sequences
Authors:MOHD AKRAM  R K JAIN   VIKAS CHAUDHARY  Y S AHLAWAT  S M PAUL KHURANA
Affiliation:Unit of Plant Virology, Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi 110 012, India;Central Potato Research Institute, Shimla 171 001, India
Abstract:The nucleotide and amino acid sequences of the movement protein (NSm) genes of five isolates of Groundnut bud necrosis virus (GBNV) originating from different hosts and parts of India such as cowpea and tomato from Kerala, groundnut from Tamil Nadu, and potato from Madhya Pradesh and Rajasthan were determined and compared to the known NSm sequences. Sequence analysis revealed that the NSm genes of GBNV isolates were identical in length (924 bp encoding 307 amino acids). GBNV isolates shared maximum identity (98–100%) at amino acid levels with GBNV‐Type isolate, while 82–83% and 34–65% amino acid sequence identities were observed with Watermelon silver mottle virus and other Tospoviruses respectively. The NSm genes among GBNV isolates originating from different hosts and locations appeared highly conserved (93–100%), suggesting their common origin.
Keywords:Groundnut bud necrosis virus    NSm gene    tospovirus
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