| 云南五个不同地区烟草花叶病毒外壳蛋白基因的序列比较 |
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| 引用本文： | 丁铭 方琦 张丽珍 杨录明 汪继玲 李滟芳 张仲凯. 云南五个不同地区烟草花叶病毒外壳蛋白基因的序列比较[J]. Virologica Sinica, 2004, 19(1): 76-78 |
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| 作者姓名： | 丁铭 方琦 张丽珍 杨录明 汪继玲 李滟芳 张仲凯 |
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| 作者单位： | [1]云南省农业科学院生物技术研究所，云南昆明650223 [2]云南红河卷烟厂，云南弥勒652300 |
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| 基金项目： | 国家烟草专卖局科技项目(110200001006B)，红河烟厂科技项目(HY2001TCS003) |
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| 摘 要： | Five Tobacco mosaic virus isolates, obtained from tobacco leaves showing typical symptom in Qujing, Honghe, Dali, Chuxiong and Yuxi in Yunnan province, were selected and studied from 637 TMV samples. Using a pair of primers specific for TMV-U1 strain, a specific fragment of 530bp including the TMV coat protein gene was amplified using IC-PCR. The products of PCR were cloned and sequenced. The nucleotide and amino acid sequences of the coat protein of the five isolates were found to be very similar each other and have more than 90% sequence identity with TMV-U1, TMV-B,TMV-P, TMV-FUJIAN, although minor differences existed among them. The results showed that the five isolates from Yunnan province belong toTMV-U1 strain.
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| 关 键 词： | 烟草花叶病毒 外壳蛋白基因 克降 序列分析 |
Comparision of the Coat Protein Gene Sequnce of Tobacco mosaic virus Isolated from Different Regions in Yunnan Province |
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| DING Ming,FANG Qi,ZHANG Li-zhen,YANG Lu-ming,Wang Ji-ling,LI Yan-fang,ZHANG Zhong-kai. Comparision of the Coat Protein Gene Sequnce of Tobacco mosaic virus Isolated from Different Regions in Yunnan Province[J]. 中国病毒学(英文版), 2004, 19(1): 76-78 |
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| Authors: | DING Ming FANG Qi ZHANG Li-zhen YANG Lu-ming Wang Ji-ling LI Yan-fang ZHANG Zhong-kai |
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| Affiliation: | DING Ming1,FANG Qi1,ZHANG Li-zhen1,YANG Lu-ming2,Wang Ji-ling2,LI Yan-fang2,ZHANG Zhong-kai1** |
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| Abstract: | Five Tobacco mosaic virus isolates, obtained from tobacco leaves showing typical symptom in Qujing, Honghe, Dali, Chuxiong and Yuxi in Yunnan province, were selected and studied from 637 TMV samples. Using a pair of primers specific for TMV-U1 strain, a specific fragment of 530bp including the TMV coat protein gene was amplified using IC-PCR. The products of PCR were cloned and sequenced. The nucleotide and amino acid sequences of the coat protein of the five isolates were found to be very similar each other and have more than 90% sequence identity with TMV-U1, TMV-B, TMV-P, TMV-FUJIAN, although minor differences existed among them. The results showed that the five isolates from Yunnan province belong toTMV-U1 strain. |
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| Keywords: | Tobacco mosaic virus CP gene Cloning Sequence analysis |
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