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α-Amylase production is induced by sulfuric acid in rice aleurone cells
Authors:Shin-ichiro Mitsunaga   Midori Kobayashi   Satoe Fukui   Kayoko Fukuoka   Osamu Kawakami   Junji Yamaguchi   Masahiro Ohshima  Toshiaki Mitsui
Affiliation:Faculty of Education, Joetsu University of Education, 1 Yamayashiki, Joetsu, Niigata, Japan. mitunaga@juen.ac.jp
Abstract:The hydrolytic enzyme alpha-amylase (EC 3.2.1.1) is produced mainly in aleurone cells of germinating cereals, and the phytohormone gibberellin (GA) is essential for its induction. However, in rice (Oryza sativa L.), sulfuric acid (H(2)SO(4)) induces alpha-amylase production in aleurone tissue even in the absence of GA. Here, the pre-treatment of rice aleurone cells with H(2)SO(4) and incubation in water induced alpha-amylase activity, as if the cells had been incubated in GA solution.
Keywords:Rice   α  -Amylase   Aleurone cells   Gibberellin   Sulfuric acid
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