A new isolation procedure and further characterisation of the lectin from the red marine alga Ptilota serrata |
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Authors: | Alexandre H Sampaio David J Rogers Clive J Barwell Silvana Saker-Sampaio Francisco H F Costa Márcio V Ramos |
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Institution: | (1) Department of Fishing Engineering, BioMol Lab, Federal University of Ceará, P.O. Box 6033, 60451-970 Fortaleza, Ceará, Brazil;(2) School of Pharmacy and Biomedical Sciences, University of Portsmouth, St Michael's Building, White Swan Road, Portsmouth, PO1 2DT, U.K.;(3) Department of Biology, BioMol Lab, Federal University of Ceará, Fortaleza, Ceará, Brazil |
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Abstract: | Ptilota serrata has been shown previously to contain a lectin (PSL) which is non-specific for human blood groups. We report here a new isolation
procedure for PSL using a single step affinity chromatography technique on cross-linked guar gum and further characterisation
studies. PSL was inhibited by galactose and its derivatives. The carbohydrates o-nitrophenyl-N-acetyl-α-D-galactoside, p-nitrophenyl-N-acetyl-β-D-galactoside and lactose were strong inhibitors. The glycoproteins porcine stomach mucin, asialo bovine mucin and asialofetuin
were also inhibitory. The Mr of PSL, determined by gel filtration, was 55,470. SDS-PAGE revealed one single protein band with
Mr of 18,390, indicating the native protein to be a trimer of apparently identical subunits. PSL was shown to contain large
amounts of acidic and hydroxyl amino acids but low in basic amino acids.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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Keywords: | Ptilota serrata red marine alga lectin agglutinin affinity chromatography guar gum sugar inhibition |
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