Sequence of puff formation inRhynchosciara polytene chromosomes

The chief characteristics of the life cycle ofRhynchosciara sp. are: egg stage (12 days); three larval instars of approximately 6 days each, followed by a 4th instar of approximately 40 days duration; pupation (6 days); and adult form (5–6 days). Maps of the 4 polytene chromosomes ofRhynchosciara sp. have been prepared, and the temporal sequence of puff formation on the chromosomes described. The cocoon is synthesized during the prepupal period, and at this time major puffs are seen on all chromosomes. The largest and most numerous puffs occur on the salivary gland chromosomes during the 24 hours prior to the last or prepupal molt. Three of the puffs that occur at this time are DNA-puffs (Summary see p. 249). Research sponsored by the U.S. Atomic Energy Commission under contract with the Union Carbide Corporation.