Institution: | 1. School of Life Science, University of Science and Technology of China, Hefei 230026, China;Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China 2. Agriculture College, Yangzhou University, Yangzhou 225002, China 3. School of Life Science, University of Science and Technology of China, Hefei 230026, China 4. Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China |
Abstract: | Application and functional study of dwarf and semi-dwarf genes are of great importance to both crop breeding and molecular biology. A new semi-dwarf gene, sd-t(t), non-allelic to sd-1, had been identified in an indica rice variety, Aitaiyin 2. In this study the gene was genetically mapped by using an F2 population, which consisted of 474 individuals developed from a cross between Aitaiyin 2 and B30. The sd-t(t) gene was located between the RFLP markers R514 and R1408B with a distance of 1.1 cM to R514, and 4.5 cM to R1408B on chromosome 4. A physical contig covering the sd-t(t) mapping region was further constructed by screening a BAC library with R514 and R1408B as probes, and the physical distance between R514 and R1408B was estimated at approximately 147 kb. This result will facilitate map-based cloning of the sd-t(t) gene. |