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Exogenous Spermidine Promotes Somatic Embryogenesis of Cunninghamia lanceolata by Altering the Endogenous Phytohormone Content
Authors:Dandan Wang  Yulin Guo  Xiaofei Long  Yan Pan  Dingjie Yang  Rui Li  Ye Lu  Ying Chen  Jisen Shi  Jinhui Chen
Affiliation:1 Key Laboratory of Forest Genetics & Biotechnology of Ministry of Education of China, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, 210037, China2 Jiangsu Key Laboratory for Poplar Germplasm Enhancement and Variety Improvement Nanjing Forestry University, Nanjing, 210037, China# These authors contributed equally: Dandan Wang, Yulin Guo
Abstract:In order to study how exogenous hormones in C. lanceolata(gymnosperm) regulate somatic embryogenesis, we measured the endogenous phytohormones of two genotypes with different somatic embryogenesis efficiency and found that an increase in endogenous concentrations of IAA and ABA may be correlated to more efficient somatic embryogenesis. By applying exogenous spermidine, we found that exogenous hormones may affect somatic embryogenesis efficiency through affecting the endogenous phytohormonecontent. Based on these results, further studies can be conducted whereby the concentration of exogenous hormones or the levels of endogenous phytohormones by molecular methods are regulated to promote somatic embryogenesis. Our research may benefit the long-term economic output of the forestry industry and lays the foundation to studying the molecular mechanismthat controls somatic embryogenesis efficiency.
Keywords:Spermidine  C. lanceolata"   target="  _blank"  >C. lanceolata  somatic embryogenesis  endogenous phytohormones
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