Morphology,Phylogeny and Culture Characteristics of <i>Ganoderma gibbosum</i> Collected from Kunming,Yunnan Province,China |
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Authors: | Thatsanee Luangharn Samantha C Karunarathna Peter E Mortimer Kevin D Hyde Jianchu Xu |
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Institution: | 1 Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China2 University of Chinese Academy of Sciences, Beijing, 100049, China3 East and Central Asia Regional Office, World Agroforestry Centre (ICRAF), Kunming, 650201, China4 Centre for Mountain Futures, Kunming Institute of Botany, Kunming, 650201, China5 Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, 57100, Thailand |
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Abstract: | Ganoderma is a genus of medicinally and economically important
mushrooms in the family Ganodermataceae. Ganoderma species are popular medicinal mushrooms and their health benefits are well-documented. Ganoderma is a
cosmopolitan genus that is widely distributed in both tropical and temperate
regions. This genus is characterized by its unique laccate or non-laccate species
with double-walled basidiospores. Here, we report on eight collections of G. gibbosum collected during surveys in Kunming, Yunnan Province, China. The specimens are described and illustrated based on macro- and micro-morphological
characteristics. Total DNA of the eight G. gibbosum strains were extracted using
the Biospin Fungal Extraction Kit following manufacturer protocol. Amplification
of the Internal Transcribed Spacer (nrITS) region was carried out using ITS5/ITS4
primers and LROR/LR5 for the nuclear ribosomal large subunit 28S rDNA gene
(LSU). Phylogenetic analysis with closely related species to G. gibbosum showed
that all eight collections grouped with G. gibbosum with 100% bootstrap support.
Phylogenetic similarity and morphological variations within the eight collections
of G. gibbosum are discussed. |
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Keywords: | Chinese medicine medicinal mushroom morphology phylogeny |
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