薄荷和罗勒提取物体外抗氧化及保肝活性研究

采用国际常用的ABTS和DPPH方法评价唇形科植物薄荷(Mentha haplocalyx) 和罗勒(Ocimum basilicum)水提物和乙醇提取物体外抗氧化活性，选用HepG2细胞，通过乙醇诱导损伤，构建体外酒精性肝损伤细胞模型，以细胞存活率评价薄荷和罗勒提取物的保肝效果。结果表明，DPPH自由基清除能力大小依次为薄荷醇提物、薄荷水提物、罗勒醇提物、罗勒水提物，半效应浓度(EC₅₀)分别为70.42 μg·mL^-1、75.77 μg·mL^-1、354.87 μg·mL^-1、451.53 μg·mL^-1。ABTS自由基清除能力大小依次为薄荷醇提物、罗勒醇提物、薄荷水提物、罗勒水提物，EC50值分别为9.04 μg·mL^-1、18.03 μg·mL^-1、22.18 μg·mL^-1、36.88 μg·mL^-1。薄荷提取物的抗氧化能力整体优于罗勒提取物，醇提物抗氧化能力优于水提物。酒精性肝损伤细胞活性检测表明，5、10、20、50 μg·mL^-1薄荷水提物、薄荷醇提物和罗勒水提物能剂量依赖性地提高乙醇诱导损伤的细胞存活率，具有一定的保肝活性。

Antioxidant and Liver Protective Effect of Mentha haplocalyx and Ocimum basilicum Extracts

ABTS and DPPH methods were used to evaluate antioxidant activity of Mentha haplocalyx and Ocimum basilicum extracts. Ethanol was used to induce damage of HepG2 cells. The survival rates of HepG2 cells after treatment with extracts were used to evaluate the liver protective effect. The results showed that the order of DPPH free radicals scavenging ability was ethanol extract of M. haplocalyx, water extract of M. haplocalyx, ethanol extract of O. basilicum, water extract of O. basilicum, and the EC50 values were 70.42 μg·mL^-1, 75.77 μg·mL^-1, 354.87 μg·mL^-1 and 451.53 μg·mL^-1, respectively. The order of ABTS free radicals scavenging ability was ethanol extract of M. haplocalyx, ethanol extract of O. basilicum, water extract of M. haplocalyx, water extract of O. basilicum, and the EC₅₀ values were 9.04 μg·mL^-1, 18.03 μg·mL^-1, 22.18 μg·mL^-1 and 36.88 μg·mL^-1, respectively. The antioxidant capacity of M. haplocalyx extract was better than O. basilicum extract and the ethanol extract was better than water extract. The cell viability assay of alcoholic liver injury showed that the survival rates of the 5, 10, 20, 50 μg·mL^-1 treatment groups of water extract of M. haplocalyx, ethanol extract of M. haplocalyx and water extract of O. basilicum were significantly higher than the ethanol injury group (eth) and showed significant liver protective effect.