诱导多能干细胞来源的肝细胞在HCV感染模型中的应用*

诱导多能干细胞(induced pluripotent stem cells,iPSCs)与胚胎干细胞(embryonic stem cells,ESCs)类似,是一类具有自我更新和无限增殖潜能的细胞, 并且能诱导分化为机体各胚层所有类型的细胞。因iPSCs来源于机体本身,规避了ESCs的免疫排斥和医学伦理等问题,具有极大的研究前景及应用潜能。大量研究表明,诱导多能干细胞分化的肝样细胞(iPS-derived hepatocyte-like cells,iHLCs)已广泛运用于HCV体内外感染模型的建立,并用于研究HCV的发病机制、宿主基因在HCV致病机制和筛选新型抗HCV药物及疫苗的研发。主要对iPSCs的来源、从不同策略诱导iPSCs成为功能性肝细胞的研究方法及其在HCV感染模型中的应用进行归纳总结。

Application of Hepatocyte-like Cells Derived from Induced Pluripotent Stem Cells in HCV Infection Model

Induced pluripotent stem cells (iPSCs) as well as embryonic stem cells(ESCs),is a kind of cells with potential of self-renewing and unlimited proliferation,has the potentian of inducing differentiation into all types of cells in every germ layer of the body. iPSCs are derived from the body itself, which avoids the immune rejection and medical ethics problems of ESCs and they play an important role in biomedical research. At present, iPS-derived hepatocyte-like cells(iHLCs) have been widely used in the establishment of in vitro and in vivo infection model of HCV.The HCV model based on iHLCs can be used to study the pathogenesis of HCV,the role of host genes in the pathogenesis of HCV, to screen new anti-HCV drugs and development of vaccine.The origin of iPSCs, the research methods of inducing iPSCs into functional liver cells by different strategies, and the application of iPSCs in HCV infection model were summarized.