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Temporal and Quantitative Expression of the Myelin-Associated Lipids,Ethanolamine Plasmalogen,Galactocerebroside, and Sulfatide,in the Differentiating CG-4 Glial Cell Line
Authors:Bichenkov  Evgeny  Ellingson  John S
Institution:(1) Department of Pathology, Anatomy, and Cell Biology, Medical College of Thomas Jefferson University, Philadelphia, PA, 19107;(2) Department of Pathology and Cell Biology, Thomas Jefferson University, Philadelphia, PA, 19107
Abstract:We determined the expression of three myelin-typical lipids in the continuous CG-4 glial cell line of oligodendrocyte progenitor cells, as the cells differentiated into oligodendrocytes. On 6 different days during the first 9 days of oligodendrocyte development, cells were labeled for 24 h with 3H]ethanolamine to label ethanolamine plasmalogens or with 3H]galactose to label the galactocerebroside and sulfogalactocerebroside; and the amount of labeled lipid expressed on each day was determined. Each labeled lipid was expressed with its own specific time course and in a defined amount on each day of differentiation. Increased labeling of plasmalogens and sulfogalactocerebroside started at early developmental stages, and increased labeling of galactocerebroside started at later stages. The results indicate that the differentiating CG-4 cell line provides a valuable system to investigate factors affecting the early time course of myelin-lipid expression and the amounts expressed.
Keywords:Galactocerebroside  plasmalogens  sulfatide  oligodendrocytes  myelin  CG-4 cells
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