A comparison of esterolytic mechanisms of several sulfhydryl proteases |
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| Authors: | KR Lynn NA Clevette-Radford |
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| Institution: | Division of Biological Sciences, National Research Council, 100 Sussex Drive, Ottawa K1A OR6, Canada |
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| Abstract: | A comparison was made of the esterolysis reactions catalyzed by chymopapain, papaya peptidases A and B, several asclepains of both the A and B families, ficin, and bromelain. Michaelis parameters for a series of aryl mesyl glycinates were measured and plotted versus the relevant Hammett σ values. All enzymes exhibited the same response to the substrates used. |
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