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Inability of tributyltin-induced chloride/hydroxyl exchange to stimulate calcium transport in mitochondria isolated from flight muscle of the sheep blowfly Lucilia cuprina
Authors:Fyfe L Bygrave and  Robyn L Smith
Institution:Department of Biochemistry, Faculty of Science, Australian National University, Canberra, A.C.T. 2600, Australia
Abstract:Tributyltin in the concentration range 1–4μm failed to stimulate Ca2+ transport by Lucilia flight-muscle mitochondria in a medium containing KCl and respiratory substrate but devoid of Pi, despite its promotion of a rapid Cl/OH exchange. When 2mm-Pi was present, concentrations of tributyltin greater than 1μm inhibited the initial rate of Ca2+ transport and induced efflux of the ion from the mitochondria in Cl- or NO3-containing media. Lower concentrations had little effect. Oligomycin added at up to 10μg/mg of mitochondrial protein had no effect on Ca2+ transport. By contrast, approx. 0.3μm-tributyltin completely inhibited respiration supported by α-glycerophosphate in either the presence or absence of added ADP. The data suggest that tributyltin can inhibit Ca2+ transport in Lucilia flight-muscle mitochondria other than by facilitating a Cl/OH exchange or producing an oligomycin-like effect.
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