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In vitro propagation of endangered Lippia filifolia mart. and schauer ex schauer |
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| Authors: | Paulo Henrique Pereira Peixoto Fátima Regina Gonçalves Salimena Marcelo de Oliveira Santos Leonardo da Silva Garcia Patrícia Maria de Oliveira Pierre Lyderson Facio Viccini Wagner Campos Otoni |
| Affiliation: | 1. Departamento de Botanica, ICB, UFJF, MG 36.036-900, Juiz de Fora, Brazil 2. Departamento de Biologia, ICB, UFJF, MG 36.036-900, Juiz de Fora, Brazil 3. Departamento de Biologia Vegetal, CCB, UFV, MG 36.570-000, Vi?osa, Brazil |
| Abstract: | Summary This work describes an efficient micropropagation protocol of Lippia filifolia. Nodal segments cultivation in MS medium supplemented with 6-benzylaminopurine (4.5 μM)/α-naphthaleneacetic acid (NAA; 54nM) induced multiple shoots (in average 27 shoots per explant). Elongated shoots were rooted with NAA (0.11 μM) and they maintained ploidy level of the in vitro produced explants. The basic chromosome number were 2n=2x=24. Regenerated rooted shoots were successfully acclimatized under shading house conditions. This is the first report involving the establishment of a protocol for shoot multiplication and rooting for endangered L. filifolia, contributing for germplasm preservation of this species. |
| Keywords: | Chromosome stability conservation medicinal plant micropropagation Verbenaceae |
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