Interrelations between oxidative stress and calcineurin in the attenuation of cardiac apoptosis by eugenol |
| |
Authors: | Rashmi Choudhary Kaushala Prasad Mishra Chivukula Subramanyam |
| |
Institution: | (1) Department of Biochemistry, Osmania University, Hyderabad, India;(2) Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Mumbai, India;(3) Department of Biochemistry, Osmania University, Hyderabad, 500 007, India |
| |
Abstract: | In view of the known involvement of oxidative stress and calcineurin (Ca2+-calmodulin dependent protein phosphatase) in β-Adrenergic stimulated events, we examined the influence of eugenol (an antioxidant
generally regarded as safe by the Food and Agricultural Organization of the United Nations) on isoproterenol-induced apoptosis
in neonatal cardiomyocytes. In comparison to unstimulated controls, cardiomyocytes stimulated with 50 μM isoproterenol for
48 h demonstrated (a) increased intracellular Ca2+ levels (b) oxidative stress involving enhanced reactive oxygen species, decreased GSH/GSSG ratio, enhanced lipid peroxidation,
increased activities of superoxide dismutase and glutathione peroxidase (c) apoptosis, evidenced by increased number of annexin
V/TUNEL positive cells, enhanced membrane fluidity, decreased mitochondrial membrane potential, increased activities of caspase
3 and 9 along with (d) increased calcineurin activity. Pre-incubation of cardiomyocytes with 100 μM eugenol for 1 h, followed
by isoproterenol treatment for 48 h, led to reversal of enhanced intracellular Ca2+ levels, oxidative stress, calcineurin activation and apoptosis caused by isoproterenol. In addition, similar treatment of
cardiomyocytes with 10 nM FK506, a calcineurin inhibitor, could also attenuate isoproterenol-induced apoptosis. These results
indicate the beneficial effects of eugenol in preventing cardiomyocyte apoptosis. |
| |
Keywords: | antioxidants apoptosis calcineurin cardiomyocytes eugenol oxidative stress |
本文献已被 PubMed SpringerLink 等数据库收录! |
|