三色拟迷孔菌Daedaleopsis tricolor抗真菌活性成分的研究

在对多孔菌发酵产物进行的活性筛选中,发现三色拟迷孔菌具有抗白假丝酵母、酿酒酵母、烟曲霉、黄曲霉等目标真菌活性。通过应用萃取、柱层析及HPLC等分离手段对其活性组分进行分离纯化,共获得3个化合物。根据波谱数据,化合物1-3的结构分别被鉴定为4-乙二醇基-8-羟基异香豆素、4-(2-羟乙酰基)-8-羟基异香豆素及4-乙二醇基-5,8-二羟基异香豆素。这3个异香豆素首次从拟迷孔菌属中分离得到,且化合物2为其活性成分。

The antifungal components from Daedaleopsis tricolor

Through screening antifungal components from secondary metabolites of polypores, a strain of Daedaleopsis tricolor was found to produce metabolites that showed antifungal activities against Candida albicans AS2.2086, Saccharomyces cerevisiae AS2.1882, Aspergillus flavus AS3.0951 and Aspergillus fumigatus AS3.5835. The solid culture of D. tricolor was extracted with methyl ethyl ketone and the extracts were fractionated by vacum liquid chromatography, Sephadex LH-20 and HPLC. Three isocoumarins (1-3) were identified as 4-(2-hydroxyethanol)-8-hydroxyisocoumarin, 4-(2-hydroxyacetyl)-8-hydroxyisocoumarin and 4-(2-hydroxyacetyl)-5, 8-hydroxyisocoumarin by analyses of their ESIMS and NMR data. These compounds were isolated for the first time from the genus Daedaleopsis and compound 2 showed antifungal activity.