Site-specific recombination of the circular 2 microns-like plasmid pKD1 requires integrity of the recombinase gene A and of the partitioning genes B and C. |
| |
| Authors: | M M Bianchi |
| |
| Institution: | Department of Cell and Developmental Biology, University of Rome, La Sapienza, Italy. |
| |
| Abstract: | In the circular plasmid pKD1, which stably replicates in Kluyveromyces lactis, the three open reading frames encode a site-specific recombinase (gene A) and two proteins involved in mitotic stability (genes B and C). A recombination analysis of plasmids in which gene B or C is inactivated reveals that unlike the 2 microns plasmid of Saccharomyces cerevisiae, these genes are also required for the site specificity of plasmid recombination. |
| |
| Keywords: | |
|
|
