Signaling pathways implicated in alpha-melanocyte stimulating hormone-induced lipolysis in 3T3-L1 adipocytes |
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Authors: | Cho Kyung-Joo Shim Jung-Hyun Cho Min-Chul Choe Yong-Kyung Hong Jin-Tae Moon Dong-Chul Kim Jong-Wan Yoon Do-Young |
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Affiliation: | Laboratory of Cell Biology, Korea Research Institute of Bioscience and Biotechnology, Yuseong, Daejeon 305-600, Korea. |
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Abstract: | Melanocortins, besides their central roles, have also recently been reported to regulate adipocyte metabolism. In this study, we attempted to characterize the mechanism underlying alpha-melanocyte-stimulating hormone (MSH)-induced lipolysis, and compared it with that of the adrenocorticotrophin hormone (ACTH) in 3T3-L1 adipocytes. Similar to ACTH, MSH treatment resulted in the release of glycerol into the cell supernatant. The activity of hormone-sensitive lipase, a rate-limiting enzyme, which is involved in lipolysis, was significantly increased by MSH treatment. In addition, a variety of kinases, including protein kinase A (PKA) and extracellular signal-regulated kinase (ERK) were also phosphorylated as the result of MSH treatment, and their specific inhibitors caused a reduction in MSH-induced glycerol release and HSL activity, indicating that MSH-induced lipolysis was mediated by these kinases. These results suggest that PKA and ERK constitute the principal signaling pathways implicated in the MSH-induced lipolytic process via the regulation of HSL in 3T3-L1 adipocytes. |
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Keywords: | melanocortins adipocyte MSH ACTH lipolysis hormone‐sensitive lipase |
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