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A novel tyrosine-phosphorylated protein inhibiting the growth of Streptomyces cells
Authors:Takanori Kumagai  Wakako Watanabe  Yasuyuki Matoba
Institution:a Department of Molecular Microbiology and Biotechnology, Graduate School of Biomedical Sciences, Hiroshima University, Kasumi 1-2-3, Minami-ku, Hiroshima 734-8551, Japan
b Frontier Center for Microbiology, Hiroshima University, Hiroshima, Japan
Abstract:Very few of the tyrosine-phosphorylated proteins in Streptomyces have been identified. Here, we identify a tyrosine-phosphorylated protein from Streptomyces coelicolor A3(2), designated as SCO5717. The protein possesses Walker motifs and a tyrosine cluster at the C-terminus. When sco5717 harboring its own promoter was introduced into the S. coelicolor cell, the growth was inhibited. An sco5717-disrupted mutant formed aerial mycelium earlier than the wild-type strain, suggesting that SCO5717 controls the cell growth of S. coelicolor. Although the recombinant SCO5717 showed an ATPase activity, it lacked self-phosphorylation ability, suggesting that SCO5717 is a novel tyrosine-phosphorylated protein, which is distinguishable from bacterial protein tyrosine kinases known so far.
Keywords:Tyrosine phosphorylation  Immunoprecipitation  Gene disruption  ATPase  Streptomyces
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