Membrane mutation related to the production of extracellular -amylase and protease in bacillus subtilis |
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Authors: | Y Yoneda K Yamane B Maruo |
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Institution: | Institute of Applied Microbiology, The University of Tokyo, Bunkyo-ku, Tokyo, Japan |
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Abstract: | Mutants which had a genetic character to increase the production of both α-amylase and protease simultaneously, were isolated from a transformable strain of Marburg by NTG treatment. This mutation seems to have occurred at a single gene of the bacterial chromosome and was not linked to 116 which was closely linked to the α-amylase gene. When this mutation and an α-amylase regulator gene (h) coexisted in one strain, their synergistic effect on extracellular α-amylase production ws observed. The introduction of this mutation resulted in a loss of competence for the transformation. The SDS disc gel electrophoretic profiles of the membrane proteins from the original strain, the mutants and transformants with this mutation showed a remarkable difference in one component. |
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