Construction of a gene bank and identification of the ribulose bisphosphate carboxylase/oxygenase genes from the nutritionally versatile cyanobacterium Chlorogloeopsis fritschii |
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Authors: | D Vakeria G A Codd A M Hawthornthwaite W D P Stewart |
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Institution: | (1) Department of Biological Sciences, University of Dundee, DD1 4HN Dundee, UK;(2) Present address: Bass plc., DE14 1JZ Burton-on-Trent, UK |
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Abstract: | A gene bank of the nutritionally versatile, nitrogen-fixing cyanobacterium Chlorogloeopsis fritschii was constructed in Charon 4A. 2,800 recombinants containing 10–20 kbp C. fritschii DNA fragments were screened by Southern hybridization using probes containing the genes for the large (LSU) and small (SSU) subunits of ribulose bisphosphate carboxylase/oxygenase (RuBisCO) from Anacystis nidulans. A single recombinant plaque ( CDG1) containing a 10.9 kbp EcoR1 fragment from C. fritschii hybridized to both the LSU and SSU probes, indicating a possible linkage of these RuBisCO genes in C. fritschii. RuBisCO activity and protein were detected in CDG1 lysates of Escherichia coli. Hybridization was also obtained between C. fritschii DNA and the LSU probe from Chlamydomonas reinhardtii, although no homology was detected using the LSU probe from maize or the SSU probe from pea.Abbreviations RuBisCO
d-ribulose 1,5-bisphosphate carboxylase/oxygenase
- RuBP
d-ribulose 1,5-bisphosphate
- LSU
large subunit of RuBisCO
- SSU
small subunit of RuBisCO
- SDS
sodium dodecyl sulphate
- DOC
deoxycholate |
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Keywords: | Chlorogloeopsis fritschii Microcystis cyanobacterial gene bank Charon 4A" target="_blank">gif" alt="lambda" align="BASELINE" BORDER="0"> Charon 4A ribulose 1 5-bisphosphate carboxylase/oxygenase |
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