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量子点-人表皮生长因子纳米荧光探针的制备及其对卵巢癌细胞SKOV-3的识别
引用本文:潘敏,储茂泉,孙烨,丁祖泉.量子点-人表皮生长因子纳米荧光探针的制备及其对卵巢癌细胞SKOV-3的识别[J].上海生物医学工程,2005,26(4):201-204.
作者姓名:潘敏  储茂泉  孙烨  丁祖泉
作者单位:[1]同济大学生命科学与技术学院,上海200092 [2]上海交通大学生命科学与技术学院,上海200032
基金项目:致谢本工作为国家自然科学基金(批准号:30300085)和上海市纳米专项(批准号:0352nm110)资助项目的部分内容.
摘    要:在连接试剂1-乙基-3(3-二甲基胺基丙基)碳二亚胺盐酸盐(1-ethyl-3(3-dimethylamino propyl)-carbodiimide,EDC)的作用下,将水相合成的量子点(Quantumdots,QDs)与人表皮生长因子(Epidermal growth factor,EGF)进行了连接,通过毛细管电泳、吸收光谱以及荧光光谱对连接前后的QDs进行了表征,获得的QDs—EGF可识别卵巢癌细胞SKOV-3。

关 键 词:量子点-人表皮生长因子  纳米荧光探针  制备方法  卵巢癌  SKOV-3细胞
收稿时间:2005-09-27
修稿时间:2005-09-27

Preparing of Quantum Dots- EGF Nanoscale Fluorescent probes and Their Specific Recognizing Ovarian Cancer Cells SKOV- 3
Pan Min, Chu MaoQuan, Sun Ye, Ding ZuQuan ;.Preparing of Quantum Dots- EGF Nanoscale Fluorescent probes and Their Specific Recognizing Ovarian Cancer Cells SKOV- 3[J].Shanghai Journal of Biomedical Engineering,2005,26(4):201-204.
Authors:Pan Min  Chu MaoQuan  Sun Ye  Ding ZuQuan ;
Institution:1. Institute of Life Sicence and Technology, Tongji University (Shanghai 200092;Shanghai 200032
Abstract:Water-soluble quantum dots (QDs) have been Successfully conjugated with epidermal growth factor (EGF) using 1-ethyl-3(3- dimethylamino propyl)-carbodiimide (EDC) as a cross-linking reagent. The properties of the QDs and QDs-EGF conjugates were characterized by capillary electrophoresis (CE), UV-Vis absorption and photoluminescent (PL) spectra. The QDs-EGF conjugates obtained could recognize the ovarian cancer cells SKOV-3.
Keywords:Quantum dots  Epidermal growth factor  Capillary electrophoresis  Optical properties  Ovarian tumor cells  Recognition
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