Homology of AFLP products in three mapping populations of barley |
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| Authors: | R Waugh N Bonar E Baird B Thomas A Graner P Hayes and W Powell |
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| Institution: | (1) Department of Cell and Molecular Genetics, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, Scotland, UK e-mail: rwaugh@scri.sari.ac.uk, GB;(2) Institute for Resistance Genetics, Federal Centre for Breeding Research on Cultivated Plants, Graf-Seinsheim-Str. 23, D-85461 Grünbach, Germany, DE;(3) Oregon State University, Corvallis, OR 97331, USA, US |
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| Abstract: | Segregation of 850 polymorphic AFLP (amplified fragment length polymorphism) fragments was followed in three different doubled
haploid (DH) barley populations, Dicktoo × Morex (DM), Igri × Franka (IF) and Blenheim × E224/3 (BE), which had previously
been used to construct linkage maps using other molecular markers. The final maps consisted of 310, 655 and 474 markers, of
which 234, 194 and 376, respectively, were AFLPs. A comparison of profiles from the parental lines identified 51 similar-sized
AFLPs segregating in both DM and IF populations, 20 in the DM and BE populations and 18 in the IF and BE populations. Eight
segregated in all three. Analysis of the complete datasets for each of the populations using Joinmap V.2. indicated that in
general terms each of the AFLPs which were polymorphic in more than one population mapped to the same genetic locus. The number
of co-dominant markers segregating in a single population ranged from 6% for DM to 12.6% for IF. These results are discussed
in the context of using AFLP in genetic linkage and diversity studies.
Received: 5 November 1996 / Accepted: 10 March 1997 |
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| Keywords: | AFLP Barley Product homology Linkage maps |
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