Characterization of a polypeptide associated with coated vesicles and the cytoskeleton which is recognized by a CREST serum |
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| Authors: | D J Bower P Jeppesen |
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| Institution: | 1. Institute of Molecular Medicine, RWTH Aachen University, Aachen, Germany;2. Department of Pathology, NYU Grossman School of Medicine, New York University, New York, USA;3. Translational Immunology Center, NYU Grossman School of Medicine, New York University, New York, USA;1. Institut de Biologia Evolutiva (CSIC-Universitat Pompeu Fabra), Barcelona, Spain;2. Department of Evolutionary Anthropology, University of Vienna, 1030 Vienna, Austria;3. Human Evolution and Archaeological Sciences (HEAS), University of Vienna, 1030 Vienna, Austria;4. Max Planck Institute for Evolutionary Anthropology, Deutscher Platz 6, 04103 Leipzig, Germany;5. Dipartimento di Biologia, Università di Roma “Tor Vergata”, Rome, Italy;6. UCL Genetics Institute, Department of Genetics, Evolution and Environment, University College London, London, UK;7. School of Biological Sciences, The University of Adelaide, Adelaide, Australia;8. German Centre for Integrative Biodiversity Research (iDiv), Halle-Jena-Leipzig, Puschstrasse 4, 04103 Leipzig, Germany;9. University of Fribourg, Fribourg, Switzerland;10. Jane Goodall Institute Spain and Senegal, Dindefelo Biological Station, Dindefelo, Kedougou, Senegal;11. Department of Wildlife and Range Management, Faculty of Renewable Natural Resources, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana;12. Fundación Rainfer, Fuente el Saz de Jarama, Madrid, Spain;13. Depts of Anthropology & Sociology and Psychology, West Chester University, West Chester, PA, USA;14. Wild Chimpanzee Foundation (WCF), Deutscher Platz 6, 04103 Leipzig, Germany;15. Wildlife Conservation Society (WCS), 2300 Southern Boulevard, Bronx, NY 10460, USA;16. Antwerp Zoo Foundation-KMDA, Royal Zoological Society of Antwerp, Antwerp, Belgium;17. Research Department, Fundació MONA, Riudellots de la Selva, Girona, Spain;18. Chimbo Foundation, Huningspaed 6. 8567 LL Oudemirdum, the Netherlands;19. The Elephant Listening Project, K. Lisa Yang Center for Conservation Bioacoustics, Cornell Lab of Ornithology, Cornell University, 159 Sapsucker Woods Road, Ithaca, NY 14850, USA;20. KMDA, Centre for Research and Conservation, Royal Zoological Society of Antwerp, Koningin Astridplein 20-26, B-2018 Antwerp, Belgium;21. Serra Húnter Professor, Department of Social Psychology and Quantitative Psychology, University of Barcelona, Barcelona, Spain;22. RSPB Centre of Conservation Science, David Attenborough Building, Pembroke Street, Cambridge, CB2 3QZ, UK;23. Greater Mahale Ecosystem Research and Conservation Project (GMERC, LTD), Mpanda, Tanzania;24. Department of Anthropology, University of Victoria, BC V8P 5C2, Canada;25. School of Human Evolution and Social Change, Arizona State University, 900 Cady Mall, Tempe, AZ 85287, USA;26. Institute of Human Origins, Arizona State University, 900 Cady Mall, Tempe, AZ 85287, USA;27. Arizona State University, PO Box 872402, Tempe, AZ 85287-2402, USA;28. EcoHealth Alliance, 520 Eighth Ave, Suite 1200, New York, NY 10018, USA;29. Evolutionary Ecology group (EVECO), Campus Drie Eiken, Universiteitsplein 1, 2610 Wilrijk, Belgium;30. Serra Húnter Fellow, Departament de Psicologia, Facultat d’Educació i Psicologia, Universitat de Girona, Girona, Spain;31. Lester E. Fisher Center for the Study and Conservation of Apes, Lincoln Park Zoo, 2001 North Clark Street, Chicago, IL 60614, USA;32. Chester Zoo, Field Programmes, Cedar House, Caughall Road, Upton-by Chester, CH2 1LH, UK;33. Wild Chimpanzee Foundation (WCF), 23BP238 Abidjan, Côte d''Ivoire;34. Panthera, 8 W 40th St., New York, NY 10018, USA;35. Centres Molina, Carrer de Lorena, 61, 08042 Barcelona, Spain;36. University College London, Department of Anthropology, 14 Taviton Street, London, WC1H 0BW, UK;37. Department of Psychology, West Chester University, West Chester, PA, USA;38. Washington University in Saint Louis, Department of Anthropology, One Brookings Drive, St. Louis, MO 63130, USA;39. Wildlife Conservation Society, Congo Program, Brazzaville, Republic of Congo;40. Primate Project, Serti, Taraba State 663001, Nigeria;41. School of Biological & Environmental Sciences, Liverpool John Moores University, James Parsons Building, Byrom Street, Liverpool L3 3AF, UK;42. Terrestrial Ecology Unit (TEREC), Department of Biology, Ghent University (UGent), K.L. Ledeganckstraat 35, 9000 Ghent, Belgium;43. Department of Human Evolutionary Biology, Harvard University, 11 Divinity Avenue, Cambridge, MA 02138, USA;44. Taï Chimpanzee Project, Centre Suisse de Recherches Scientifiques, BP 1301, Abidjan 01, Côte d''Ivoire;45. Department of Geography, Planning and Environmental Studies, University of Concordia, 1455 Boulevard de Maisonneuve Ouest, Montréal, QC H3G 1M8, Canada;46. Université de Neuchâtel, Institut de Biologie, Rue Emile-Argand 11, 2000 Neuchâtel, Switzerland;47. School of Psychology and Neuroscience, University of St. Andrews, St. Andrews, UK;48. CNAG-CRG, Centre for Genomic Regulation (CRG), Barcelona Institute of Science and Technology, Barcelona, Spain;49. MRC Integrative Epidemiology Unit, University of Bristol, Bristol, UK;50. Population Health Sciences, Bristol Medical School, University of Bristol, Bristol BS8 2BN, UK;51. Institut Catala de Paleontologia Miquel Crusafont, Universitat Autonoma de Barcelona, Cerdanyola del Vallès, Spain;52. Catalan Institution of Research and Advanced Studies (ICREA), Barcelona, Spain;1. Institut für Molekulare Zellbiologie, Westfälische Wilhems-Universität Münster, Münster, Germany;2. Center for Translational Immunology, University Medical Center Utrecht, Utrecht, The Netherlands;3. Department of Physiology, Semmelweis University School of Medicine, Budapest, Hungary;4. Department of Physiology, Pathophysiology and Toxicology and ZBAF (Centre for Biomedical Education and Research), Faculty of Health, School of Medicine, Witten/Herdecke University, Witten, Germany;1. Geisinger Health System, 100 N. Academy Ave. M.C. 44-00, Danville, PA 17822, USA;2. Department of Chemistry, Susquehanna University, 517 University Ave., Selinsgrove, PA 17870, USA;1. Catalan Institute for Research and Advanced Studies, Barcelona, Spain;2. Institut Hospital del Mar d’Investigacions Mèdiques, Barcelona Biomedical Research Park, Barcelona, Spain;3. Division of Clinical Immunology, Department of Medicine, Mount Sinai School of Medicine, New York;4. Lydia Becher Institute of Immunology and Inflammation, University of Manchester, Manchester, United Kingdom |
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| Abstract: | Serum from an individual with the CREST syndrome (calcinosis, Raynaud's phenomenon, esophageal dismotility, sclerodactyly, telangiectasia) reacts not only with kinetochores, but also with a cytoplasmic, phosphorylatable polypeptide, which is shown by immunofluorescence in whole cells and immunoelectronmicroscopy in sections to be associated with actin stress fibres in cultured mammalian cells. The antigen shows some variation in molecular weight between species, estimated by immunoblotting to range from 68 to 76 kD between mouse, Chinese hamster, sheep and human cells. Much of the polypeptide copurifies with coated vesicles, of which approx. 5% bound antibody from the serum, as detected by immunogold electronmicroscopy. |
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