UBC9在人肺癌组织中高表达及其临床意义(英文)

目的:由于Ubc9在肿瘤的发生于恶化中发挥巨大作用,本研究目的是对泛素结合酶(Ubc9)mRNA和Ubc9蛋白在非小细胞肺癌组织中表达进行检测,评估Ubc9在肺癌预后中的指导意义。方法:通过荧光定量PCR、免疫组织化学法、Western-blot检测100例非小细胞肺癌病人中Ubc9 mRNA、蛋白水平的表达进一步研究Ubc9的表达与肺癌临床特征的关系。结果:实验结果显示在肺癌细胞中Ubc9阳性表达显示为黄棕色颗粒,与癌旁组织比较,Ubc9 mRNA、蛋白在肺癌组织中高表达,并且与肺癌的临床分型(分期、淋巴结转移、吸烟、分化)有关。Ubc9 mRNA、蛋白在肺癌中的表达癌组织高于癌旁、有淋巴结转移的高于无转移、吸烟高于不吸烟者、低分化组织高于高分化组织。结论:由此可见Ubc9 mRNA、蛋白水平的高表达可能对肺癌临床特征的评估,以及预测术后生存率都有重要指导意义。

Higher Expression of Ubc9 in Human Lung Cancer Tissues and Its clinical Significance*

Objective： As Ubc9 may play a great role in tumor genesis and deterioration, the purpose of this study was to detect the expression ofubiquitin-conjugating enzyme （Ubc9） and assess the prognostic significance of Ubc9 in lung cancer. Methods： The expres- sion ofUbc9 mRNA and Ubc9 protein in Non-small cell lung cancer and para-carcinoma tissue from 100 lung cancer patients were de- tected using Real-time, Immunohistochemistry and Western-blot. The correlation of Ubc9 expression with the clinical characteristics was analyzed by the single factor correlation analysis. Results： The Ubc9 protein stained brown granules in cytoplasm and nucleus of cancer cells. The higher expression of Ubc9 mRNA and Ubc9 protein were detected in cancer tissue than that in para-carcinoma tissue and cor- related with TNM stage, lymph nodes metastases, differentiation and smoking. Conclusions： The expression level ofUbc9 mRNA or pro- tein may independently predict postsurgical survival in lung cancer.