HSF1在子宫内膜癌细胞中的表达及其与抗氧化的相关性研究

目的:探讨子宫内膜癌细胞在受到过氧化氢刺激时热休克因子1(Heat Shock Factor 1,HSF1)表达的变化,以及HSF1对肿瘤细胞抗凋亡能力的影响。方法:选择子宫内膜癌的Ishikawa、HEC-1-B及RL95-2三株细胞。分别测定细胞中HSF1基因转录以及翻译表达水平。给予细胞不同浓度的H2O2刺激后,检测细胞内HSF1的mRNA表达变化并且统计受刺激后细胞受抑制情况,观察细胞存活和生长与HSF1含量的关系。结果:三株细胞中HSF1mRNA和蛋白表达的基水平不同;在受到H2O2刺激后,细胞内HSF1表达有不同程度的升高;Ishikawa、HEC-1-B细胞分别在受到较高浓度的H2O2刺激后,细胞存活率出现明显下降;而RL95-2细胞在受到相对低浓度的H2O2刺激后,细胞存活率即出现明显降低。结论:一定范围内浓度的H2O2刺激能够上调子宫内膜癌细胞中HSF1在转录以及蛋白水平的表达,而过高的浓度会使细胞中HSF1表达减少,对于不同细胞来说H2O2刺激的适宜浓度不同。而能使细胞增殖与增长发生明显变化的H2O2浓度与细胞内HSF1表达水平相关。

Heat Shock Factor-1 Expression in Different Endometrial Cancer Cells and its Effects on Tumor Cell Apoptosis Resistance

Objective： To investigate the expression of Heat Shock Factor 1 （HSF1） mRNA gene in hydrogen peroxide treated en- dometrial cancer cells, and the influence of HSF1 on tumor cell apoptosis resistance. Methods： Three human endometrial cancer cell lines, Ishikawa and HEC-1-B and RL95-2 cells were performed in this study. HSF1 mRNA gene and protein expression of three cell lines were measured. Cells were treated with different hydrogen peroxide （H2O2） concentrations. The effect on HSF1 expression, cell growth and viability was analyzed. Results： HSF1 mRNA and protein expression of three cell lines was different. HSF1 mRNA gene expression increased with varying degrees in H2O2 treated cells. In comparison, cell growth and viability of Ishikawa and HEC-1-B cells decreased significantly in higher concentration H2O2, while that of RL95-2 cells declined considerably. Conclusions： H2O2 induced different effects according to cell type, time of incubation and concentration exposure. Within a certain range of concentration, H2O2 increases HSFI ex- pression in endometrial cancer cells. However excessive H2O2 can reduce HSF1 expression in cells, and the suitable concentration of H2O2 is different for variant cells. The H2O2 concentration that can make the obvious changes in cell proliferation and growth is associated with HSF1 expression levels in the cell.